Cell Based Assays
Promega TRKA Kinase Enzyme System, 1mg
Recombinant human TRKA (amino acids 440-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TRKA is a member of the trk proto-oncogene family and encodes a 140kDa membrane-spanning protein tyrosine kinase.
Promega c-KIT Kinase Enzyme System, 10microg
Recombinant human c-KIT (544-end) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. c-KIT is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor).
Promega LRRK2 Kinase Enzyme System, 10microg
LRRK2 or leucine-rich repeat kinase is a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain and a WD40 domain.
Promega ADP-Glo™ Kinase Assay + PAK1/CDC42 Kinase Enzyme System
Recombinant full-length human PAK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag and combined with CDC42 at a ratio of 1:4 (PAK1:CDC42) in vitro. PAK1 is a member of the p21-activated kinases (PAKs).
Promega CAMK1 gamma Kinase Enzyme System, 1mg
Full-length recombinant human CAMK1 gamma was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CAMK1 gamma (CLICK-III), a member of the CAMK family, is a novel membrane-anchored neuronal Ca2+/calmodulin-dependent protein kinase.
Promega RET Kinase Enzyme System, 1mg
Recombinant human RET (amino acids 658-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. RET gene codes for a transmembrane tyrosine kinase.
Promega ADP-Glo™ Kinase Assay+ MAPKAPK2 Kinase Enzyme System
Recombinant human MAPKAPK2 (46-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. MAPKAPK2 (MAPKAP kinase2) is a Ser/Thr protein kinase that is regulated via direct phosphorylation by p38 MAP kinase.
Promega CK2 alpha 1 Kinase Enzyme System, 1mg
Recombinant full-length human CK2 alpha 1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CK2 alpha 1 is a serine-threonine protein kinase whose targets include many critical regulators of cellular growth.
Promega MAPKAPK5 Kinase Enzyme System, 1mg
Recombinant full-length human MAPKAPK5 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MAPKAPK5 is a member of the serine/threonine kinase family that responds to cellular stress and proinflammatory cytokines.
Promega PLK1 Kinase Enzyme System, 10microg
Recombinant full-length human PLK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. A member of the Polo-Like Kinase family, PLK1 localizes to centrosomes and undergoes dramatic subcellular relocation during the cell cycle.
Promega ADP-Glo™ Kinase Assay + EGFR (L858R) Kinase Enzyme System
Recombinant human EGFR (L858R) (amino acids 695-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity.
Promega ADP-Glo™ Kinase Assay + VRK2 Kinase Enzyme System
Recombinant human VRK2 (1-375) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. VRK2 (also known as vaccinia related kinase 2) is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases.
Promega CDK1/CyclinA2 Kinase Enzyme System, 1mg
Recombinant full-length human CDK1 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK1 is essential for completion of START, controlling event in the cell cycle required to initiate mitosis.
Promega PKC gamma Kinase Enzyme System, 1mg
Recombinant full-length human PKC gamma was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC gamma is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases.
Promega ADP-Glo™ Kinase Assay with PI:3PS
The ADP-Glo™ Kinase assay and lipid kinase substrates PIP2:3PS and PI:3PS are components of the PI3K-Glo™ Class I Kinase Profiling System that are available for purchase separately.